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1.
Acta Pharmaceutica Sinica ; (12): 2763-2770, 2023.
Article in Chinese | WPRIM | ID: wpr-999007

ABSTRACT

A quantitative analysis of multi-components by single marker method (QAMS) was established for simultaneous determination of gallic acid, protocatechuic acid, catechin, epicatechin, p-coumaric acid, ferulic acid and phloridzin in Cynomorium songaricum Rupr. The analysis was performed on a ChromCore Polae C18 column (250 mm×4.6 mm, 5 μm) , with a mobile phase consisting of acetonitrile-0.3% phosphoric acid aqueous solution for gradient elution. The volume flow rate, column temperature and sample injection volume were set at 1.0 mL·min-1, 25 ℃, and 40 µL, respectively. The relative correction factors of gallic acid and protocatechuic acid, catechin, epicatechin, p-coumaric acid, ferulic acid and phloridzin were calculated and the durability was also investigated. The contents of these seven compounds in fourteen batches of Cynomorium songaricum Rupr. from different producing areas or batches were determined by external standard method (ESM) and quantitative analysis of multi-components with a single-marker method (QAMS), respectively. SPSS and Origin Pro software were employed for principal components assay, similarity evaluation and cluster analysis. The specificity, precision, repeatability, stability and linear range (R2 > 0.999 0) of the seven components were all good. The average recovery was 96.89%-103.16% and RSD was 0.55%-2.76%. Then gallic acid was chosen as internal reference for calculation the correction factors for the other six components, the average relative correction factors of protocatechuic acid, catechin, epicatechin, p-coumaric acid, ferulic acid and phloridzin were 1.141 5, 0.200 5, 0.208 0, 2.361 9, 1.867 7, 0.204 6, respectively. Student's test results showed that there was no significant difference between the data analyzed by ESM and the data obtained from QAMS method. Through data visualization analysis, the contents of gallic acid, protocatechuic acid, catechin and epicatechin in different samples were significantly different, indicating that these four components might be the main quality markers of Cynomorium songaricum Rupr. for gaving more contributes to the principal components. The cluster analysis showed that samples from Xinjiang and samples from Inner Mongolia were clustered in significantly different categories, meaning that the quality of Cynomorium songaricum Rupr. had great relation with producing areas. The method of QAMS established in this study is a simple, economical and practical method with scientific and applicable charactistics for evaluating the quality of Cynomorium songaricum Rupr. efficiently and scientifically.

2.
Chinese Traditional and Herbal Drugs ; (24): 216-220, 2015.
Article in Chinese | WPRIM | ID: wpr-854322

ABSTRACT

Objective: To study the effect of processing techniques on reducing the contents of safrole and aristolochic acid A in Asari Radix et Rhizoma. Methods: The contents of safrole and aristolochic acid A between raw herb and processed products were determined by HPLC, and the differences in the contents of toxic components between raw herb and processed products were used to evaluate the detoxification efficiency. Results: The linear ranges of safrole and aristolochic acid A were 169.2-846.0 μg (r = 0.9996) and 11.6-58.0 ng (r = 0.9996), respectively. And the average recovery rates were 99.83% (RSD = 1.67%) and 101.43% (RSD = 1.25%). The removal rate of safrole in order was as follows: salt system > fried coke > rice water system > alkali > liquorice > vinegar > ginger > wine > alkali-vinegar > honey and the removal rate of aristolochic acid A was in ordor as: fried coke > alkali-vinegar > salt system > alkali > vinegar > rice water system > liquorice > wine > honey > ginger. The removal rate of aristolochic acid A in Asari Radix et Rhizoma by fried coke was over 60%. Conclusion: The contents of safrole and aristolochic acid A in Asari Radix et Rhizoma could be decreased to different extent by processing techniques. Among the processing methods, processing by fried coke is the best method.

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